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Bioengineering

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CometChip: A alta taxa de transferência de plataforma 96-Bem, para avaliação dos danos causados ​​no DNA em células Microarrayed Humanos

Article DOI: 10.3791/50607-v • 10:59 min • October 18th, 2014
October 18th, 2014 •
*1, *2, 3, 1, 1, 2, 2, 1
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota
* These authors contributed equally

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Descrevemos aqui uma plataforma que permite a detecção de teste do cometa de danos no DNA, com taxa de transferência sem precedentes. Os padrões de dispositivos de células de mamíferos para um microarranjo e permite o processamento em paralelo de amostras 96. A abordagem facilita a análise de danos ao DNA nível base, danos ao DNA induzidos por exposição e reparo do DNA cinética.

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Bioengenharia ensaio cometa eletroforese microarray danos no DNA reparo de DNA
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