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Journal / Bioengineering / Permeabilization av levd Cells Bruke en Inert Gas …
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JoVE Journal Bioengineering
Permeabilization av levd Cells Bruke en Inert Gas Jet
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Permeabilization av levd Cells Bruke en Inert Gas Jet

Article DOI: 10.3791/50612-v • 08:21 min • September 4th, 2013
September 4th, 2013 •
1, 1, 1, 1, 1, 1,2
1Chemical Engineering, McGill University, 2Montreal Heart Institute

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Denne protokollen beskriver en fremgangsmåte for midlertidig permeabilization av adherente celler ved hjelp av en inert gass-stråle. Denne teknikk muliggjør overføring av genetisk materiale og biomolekyler i adherente pattedyrceller ved anvendelse av mekaniske krefter for å forstyrre plasmamembranen.

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Bioteknologi Chemical Engineering kjemi biokjemi cellebiologi molekylær biologi Biomedical Engineering biofysikk Transfection Permeabilization transfeksjon skjærspenning heftende celler SEM celle mikroskopi bildebehandling
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