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Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

Article DOI: 10.3791/50615-v • 07:09 min • September 3rd, 2013
September 3rd, 2013 •
1,2, 1, 2,3, 3, 4
1Experimental Surgical Research, Grosshadern Hospital, Munich, 2Center for Liver Cell Research, Grosshadern Hospital, Munich, 3Hepacult LLC, Regensburg, 4Department of Surgery, Grosshadern Hospital, Munich

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Summary
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A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.

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Isolation Human Hepatocytes Two-step Collagenase Perfusion Procedure Liver Regeneration Capacity Detoxification Metabolism Homeostasis Research Questions Pharmacokinetics Toxicology Liver Regeneration Translational Research Modified Version Enzymatic Methods Structural Integrity Function Rat Livers Human Liver Pieces Yield Of Hepatocytes Viability Cannulization Of Blood Vessels Comparable Liver Or Blood Vessel Sizes
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