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JoVE Journal Bioengineering
Biosensors Ratiométrique qui mesurent l'état redox mitochondrial et de l'ATP dans les cellules vivantes de levure
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Biosensors Ratiométrique qui mesurent l'état redox mitochondrial et de l'ATP dans les cellules vivantes de levure

Article DOI: 10.3791/50633-v • 12:22 min • July 22nd, 2013
July 22nd, 2013 •
*1, *1, 2, 2, 1
1Department of Pathology and Cell Biology, Columbia University, 2Herbert Irving Comprehensive Cancer Center, Columbia University
* These authors contributed equally

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Nous décrivons l'utilisation de deux ratiométriques, biocapteurs génétiquement codés, qui sont fondés sur la GFP, pour surveiller l'état redox mitochondrial et les niveaux d'ATP à une résolution subcellulaire dans les cellules de levure vivantes.

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Bio-ingénierie Numéro 77 microbiologie biologie cellulaire biologie moléculaire biochimie sciences de la vie roGFP la protéine fluorescente verte redox-sensibles GO-ATeam ATP FRET ROS les mitochondries les biocapteurs la GFP ImageJ la microscopie la microscopie confocale cellule l'imagerie
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