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A cultura de células epiteliais nasais Humano na interface ar líquido
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A cultura de células epiteliais nasais Humano na interface ar líquido

Article DOI: 10.3791/50646-v • 10:38 min • October 8th, 2013
October 8th, 2013 •
1, 1, 1,2, 1,3, 1,2,4
1Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, 2Department of Pediatrics, The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care, The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology, The University of North Carolina at Chapel Hill

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Células epiteliais nasais, obtidas através de biópsia superficial raspar de voluntários humanos, são expandidas e transferido para tecido inserções de cultura. Ao atingir a confluência, as células são cultivadas na interface ar-líquido, obtendo-se culturas de células ciliadas e não ciliadas. Culturas de células epiteliais nasais diferenciados fornecem modelos experimentais para o estudo de viáveis ​​da mucosa respiratória.

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Biologia Celular Edição 80 Epitélio modelos de cultura celular ciliada poluição do ar os modelos de co-cultura o epitélio nasal
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