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Immunology and Infection

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Culturing Microglia from the Neonatal and Adult Central Nervous System

Article DOI: 10.3791/50647-v • 11:28 min • August 9th, 2013
August 9th, 2013 •
*1,2, *2,3, *2,3, 2
1Program in Neuroscience, Stony Brook University, 2Department of Pharmacological Sciences, Stony Brook University, 3Program in Molecular and Cellular Pharmacology, Stony Brook University
* These authors contributed equally

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Summary
Automatic Translation

We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.

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Culturing Microglia Neonatal Adult Central Nervous System Macrophage-like Cells Physiological Processes Pathological Processes CNS Maturation Multiple Sclerosis Spinal Cord Injury Neuronal Injury Axonal Damage Ischemic Brain Trauma Stroke Immunocompetent Members Synaptic Plasticity Culturing Protocols Viable Cell Numbers Confounding Variables CNS Cell Types Cell Culture Debris Cortex Spinal Cord Segments Reproducibility Alternative To Neonatal Brain Microglia Pathologies Affecting Postnatal Spinal Cord In Vitro Systems
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