Ved hjelp av en automatisert 3D-tracking system for å registrere Individuell og Shoals av Adult sebrafisk

Biology
 

Cite this Article

Copy Citation | Download Citations | Reprints and Permissions

Maaswinkel, H., Zhu, L., Weng, W. Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish. J. Vis. Exp. (82), e50681, doi:10.3791/50681 (2013).

Please note that all translations are automatically generated.

Click here for the english version. For other languages click here.

Abstract

Materials

Name Company Catalog Number Comments
AABT-3D Tracking Hardware xyZfish AABT II Includes camera, video card, observation compartment with mirror and lighting
AABT-3D Calibration equipment xyZfish calib Contains calibration panel and side mirror
AABT-3D Observation containers xyZfish cubes  
AABT-3D Tracking software xyZfish v.1.0  
Computer optional N/A Windows XP or higher
(+)-MK-801 hydrogen maleate Sigma M107  
Air pump Fusion 500  
Air line tubing 3/16” Lee’s Aquarium and Pet Products 14508  
Medium Binder Clips OfficeDepot 561339 To attach airline to observation container

DOWNLOAD MATERIALS LIST

References

  1. Brennan, C. H. Zebrafish behavioural assays of translational relevance for the study of psychiatric disease. Rev. Neurosci. 22, 37-48 (2011).
  2. Kabashi, E., Brustein, E., Champagne, N., Drapeau, P. Zebrafish models for the functional genomics of neurogenetic disorders. Biochim. Biophys. Acta. 1812, 335-345 (2011).
  3. McCollum, C. W., Ducharme, N. A., Bondesson, M., Gustafsson, J. A. Developmental toxicity screening in zebrafish. Birth. Defects. Res. C. Embryo Today. 93, 67-114 (2011).
  4. Kurta, A., Palestis, B. Effects of ethanol on the shoaling behavior of zebrafish (Danio rerio). Dose-Response. 8, 527-533 (2010).
  5. Sison, M., Gerlai, R. Behavioral performance altering effects of MK-801 in zebrafish (Danio rerio). Behav. Brain Res. 220, 331-337 (2011).
  6. Delcourt, J., Denoël, M., Ylieff, M., Poncin, P. Video multitracking of fish behaviour: a synthesis and future perspectives. Fish Fish. 14, 186-204 (2013).
  7. Zhu, L., Weng, W. Catadioptric stereo-vision system for the real-time monitoring of 3D behavior in aquatic animals. Physiol. Behav. 91, 106-119 (2007).
  8. System for 3D monitoring and analysis of motion behavior of targets. US patent. Zhu, L., Wang, J., Su, R., Weng, W. 8306260 (2012).
  9. Cachat, J., et al. Three-dimensional neurophenotyping of adult zebrafish behavior. PLoS ONE. 6, e17597 (2011).
  10. Maaswinkel, H., Zhu, L., Weng, W. The immediate and the delayed effects of buspirone on zebrafish (Danio rerio) in an open field test: a 3-D approach. Behav. Brain Res. 234, 365-374 (2012).
  11. Maaswinkel, H., Le, X., He, L., Zhu, L., Weng, W. Dissociating the effects of habituation, black walls, buspirone and ethanol on anxiety-like behavioral responses in shoaling zebrafish. A 3D approach to social behavior. Pharmacol. Biochem. Behav. 108, 16-27 (2013).
  12. Maaswinkel, H., Zhu, L., Weng, W. The advantages of a 3D-automatic recording system for the study of shoaling behavior in zebrafish. 10th International Conference Zebrafish Development and Genetics, Madison, (2012).
  13. Maaswinkel, H., Zhu, L., Weng, W. An automatic recording system to quantify antagonistic interactions between zebrafish. Abstract. 10th International Conference Zebrafish Development and Genetics, Madison, (2012).
  14. Spence, R., Gerlach, G., Lawrence, C., Smith, C. The behaviour and ecology of the zebrafish, Danio rerio. Biol. Rev. Camb. Phil. Soc. 83, 13-34 (2008).
  15. Krause, J., Hartmann, N., Pritchard, V. L. The influence of nutritional state on shoal choice in zebrafish, Danio rerio. Anim. Behav. 57, 771-775 (1999).
  16. Speedie, N., Gerlai, R. Alarm substance induced behavioral responses in zebrafish (Danio rerio). Behav. Brain Res. 188, 168-177 (2008).
  17. Gebauer, D. L., Pagnussat, N., Piato, A. L., Schaeffer, I. C., Bonan, C. D., Lara, D. R. Effects of anxiolytics in zebrafish: similarities and differences between benzodiazepines, buspirone and. 99, 480-486 (2011).
  18. Paull, G. C., Filby, A. L., Giddins, H. G., Coe, T. S., Hamilton, P. B., Tyler, C. R. Dominance hierarchies in zebrafish (Danio rerio) and their relationship with reproductive success. Zebrafish. 7, 109-117 (2010).
  19. Engeszer, R. E., Ryan, M. J., Parichy, D. M. Learned social preference in zebrafish. Curr. Biol. 14, 881-884 (2004).
  20. Mathur, P., Guo, S. Use of zebrafish as a model to understand mechanisms of addiction and complex neurobehavioral phenotypes. Neurobiol. Dis. 40, 66-72 (2010).
  21. Tropepe, V., Sive, H. L. Can zebrafish be used as a model to study the neurodevelopmental causes of autism. Genes Brain Behav. 2, 268-281 (2003).
  22. Echevarria, D. J., Hammack, C. M., Pratt, D. W., Hosemann, J. D. A novel behavioral test battery to assess global drug effects using zebrafish. Int. J. Comp. Psychol. 21, 19-34 (2008).
  23. Seibt, K. J., Piato, A. L., Luz Oliveira, da, Capiotti, R., Vianna, K. M., R, M., Bonan, C. D. Antipsychotic drugs reverse MK-801 induced cognitive and social interaction deficits in zebrafish (Danio rerio). Behav. Brain. Res. 224, 135-139 (2011).
  24. Zou, H., et al. Low dose MK-801 reduces social investigation in mice. Pharmacol. Biochem. Behav. 90, 753-757 (2008).
  25. Ng, M. C., Hsu, C. P., Wu, Y. J., Wu, S. Y., Yang, Y. L., Lu, K. T. Effect of MK-801-induced impairment of inhibitory avoidance learning in zebrafish via inactivation of extracellular signal-regulated kinase (ERK) in telencephalon. Fish Physiol. Biochem. 38, 1099-1106 (2012).
  26. Sison, M., Gerlai, R. Associative learning performance is impaired in zebrafish (Danio rerio) by the NMDA-R antagonist MK-801. Neurobiol. Learn. Mem. 96, 230-237 (2011).
  27. Swain, H. A., Sigstad, C., Scalzo, F. M. Effects of dizocilpine (MK-801) on circling behavior, swimming activity, and place preference in zebrafish (Danio rerio). Neurotox. Teratol. 26, 725-729 (2004).
  28. Vezzani, A., et al. Kinetics of MK-801 and its effect on quinolinic acid-induced seizures and neurotoxicity in rats. J. Pharmacol. Exp. Ther. 249, 278-283 (1989).
  29. Green, J., et al. Automated high-throughput neurophenotyping of zebrafish social behavior. J. Neurosci. Methods. 210, 266-271 (2012).
  30. Rung, J. P., Carlsson, A., Rydén Markinhuhta, K., Carlsson, M. L. (+)-MK-801 induced social withdrawal in rats; a model for negative symptoms of schizophrenia. Prog. Neuro-psychopharmacol. Biol. Psych. 29, 827-832 (2005).
  31. Gururajan, A., Taylor, D. A., Malone, D. T. Cannabidiol and clozapine reverse MK-801-induced deficits in social interaction and hyperactivity in Sprague-Dawley rats. J. Psychopharmacol. 226, 1317-1332 (2012).
  32. Burket, J. A., Cannon, W. R., Jacome, L. F., Deutsch, S. I. MK-801, a noncompetitive NMDA receptor antagonist, elicits circling behavior in the genetically inbred Balb/c mouse strain. Brain Res. Bull. 83, 337-339 (2010).
  33. Saunders, J. A., Gandal, M. J., Roberts, T. P., Siegel, S. J. NMDA antagonist MK801 recreates auditory electrophysiology disruption present in autism and other neurodevelopmental disorders. Behav. Brain Res. 234, 233-237 (2012).
  34. Brosda, J., et al. Pharmacological and parametrical investigation of prepulse inhibition of startle and prepulse elicited reactions in Wistar rats. Pharmacol. Biochem. Behav. 99, 22-28 (2011).
  35. Wong, K., et al. Analyzing habituation responses to novelty in zebrafish (Danio rerio). Behav. Brain Res. 208, 450-457 (2010).
  36. Maximino, C., et al. Parametric analyses of anxiety in zebrafish scototaxis. Behav. Brain Res. 210, 1-7 (2010).
  37. Blaser, R. E., Rosemberg, D. B. Measures of anxiety in zebrafish (Danio rerio): dissociation of black/white preference and novel tank test. PloS One. 7, e36931 (2012).

Comments

2 Comments

  1. Is the software released as an open source?

    Reply
    Posted by: Alex L.
    October 14, 2015 - 10:33 AM
  2. No, this is commercial software (website: www.xyZfish.com)

    Reply
    Posted by: Hans M.
    October 15, 2015 - 4:08 PM

Post a Question / Comment / Request

You must be signed in to post a comment. Please or create an account.

Usage Statistics