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Micronschaal Resolutie Optische Tomografie van Gehele Mouse Brains met confocale Light Sheet Microscopie

Article DOI: 10.3791/50696-v • 09:49 min • October 8th, 2013
October 8th, 2013 •
1, 2,3, 1, 1,4, 5, 2, 1,4,6,7
1European Laboratory for Non-linear Spectroscopy (LENS), 2Integrated Research Centre, University Campus Bio-medico of Rome, 3DAEMI, University of Cassino, 4National Institute of Optics (CNR-INO), 5Allen Institute for Brain Science, 6Department of Physics, University of Florence, 7ICON Foundation, Sesto Fiorentino, Italy

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In dit artikel beschrijven we de volledige experimentele procedure te reconstrueren, met een hoge resolutie, de fijne hersenen anatomie van fluorescent gelabelde muis hersenen. De beschreven protocol bevat monstervoorbereiding en clearing, specimen montage voor imaging, data post-processing en multi-schaal visualisatie.

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Neurowetenschappen Microscopie Neuro-anatomie connectomics licht blad microscopie Whole-brain imaging
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