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A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines
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A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

Article DOI: 10.3791/50712-v • 07:09 min • January 3rd, 2014
January 3rd, 2014 •
1, 1
1Department of Plant Pathology, University of Georgia

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Summary
Automatic Translation

The use of a needle injection method to inoculate maize and teosinte plants with the biotrophic pathogen Ustilago maydis is described. The needle injection inoculation method facilitates the controlled delivery of the fungal pathogen in between the plant leaves where the pathogen enters the plant through the formation of appresoria. This method is highly efficient, enabling reproducible inoculations with U. maydis.

Tags

Pathogenicity Assessment Ustilago Maydis Maize Teosinte Biotrophic Pathogens Corn Smut Yield Losses Crop Rotation Fungicide Application Seed Treatments Host Resistance Pathogen Inoculation Method Plant Leaves Resistance Reaction Screening Method Needle Injection Inoculation Method Maize X Teosinte Introgression Lines
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