Секс Стратифицированная нейронов культур для изучения ишемических: Подготовка гибель клеток

* These authors contributed equally
Neuroscience

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Cite this Article

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Fairbanks, S. L., Vest, R., Verma, S., Traystman, R. J., Herson, P. S. Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways. J. Vis. Exp. (82), e50758, doi:10.3791/50758 (2013).

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Abstract

Materials

Name Company Catalog Number Comments
B27 Supplement Invitrogen 17504-044
Borax Sigma 71997
Boric Acid Sigma B6768
Bovine Serum Albumin Sigma A4503
Fetal Bovine Serum Invitrogen 26140-079
GlutaMAX Invitrogen 35050-061
Glutamine Invitrogen 25030-081
HBSS, no calcium, no magnesium, no phenol red Invitrogen 14175-095
MEM, no glutamine, no phenol red Invitrogen 51200-038
Neurobasal, no glutamine, no phenol red Invitrogen 12348-017
Penicillin/Streptomycin Invitrogen 15070-063
Poly-D-lysine, MW 70,000-150,000 Sigma P0899
Trypan Blue 0.4% Invitrogen 15250-061
EQUIPMENT
Dumont #5/45 Angled Forceps Fine Science Tools 11251-35
Dumont #7 – Fine Curved Forceps Fine Science Tools 11274-20
Serrated (London) Forceps Fine Science Tools 11080-02
Extra Fine Graefe Forceps 1 x 2 teeth Fine Science Tools 11153-10
Moria Spring Scissors Fine Science Tools 15396-00
Medium Operating Scissors Roboz Surgical RS-6812
Extra Fine Microdissecting Scissors Roboz Surgical RS-5880
24-well Tissue Culture Plates Fisher 87721
Cell Strainer, 40 µM Nylon Mesh BD Biosciences 352340

DOWNLOAD MATERIALS LIST

References

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