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Journal / Biology / Isolering af microvaskulæreendothelial Tubes fra…
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Isolering af microvaskulæreendothelial Tubes fra Mouse modstandskar
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Isolering af microvaskulæreendothelial Tubes fra Mouse modstandskar

Article DOI: 10.3791/50759-v • 09:23 min • November 25th, 2013
November 25th, 2013 •
1, 1,2
1Medical Pharmacology and Physiology, University of Missouri, 2Dalton Cardiovascular Research Center

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Vi præsenterer en forberedelse til at visualisere og manipulere calcium signalering i native, intakt mikrovaskulære endotel. Endoteliale rør frisk isoleret fra mus modstand arterier forsyner skeletmuskulaturen beholde in vivo morfologi og dynamisk signalering inden for og mellem de omkringliggende celler. Endoteliale rør kan fremstilles ud fra mikrokar i andre væv og organer.

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Grundlæggende protokol endotel rør mikrocirkulationen calcium signalering modstand vaskulatur konfokal mikroskopi
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