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Journal / Biology / Protokoller for å gjennomføre en
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Biology

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Protokoller for å gjennomføre en

Article DOI: 10.3791/50762-v • 16:11 min • September 16th, 2013
September 16th, 2013 •
*1, *2, 3, 4, 2, 4
1Department of Biology, California Institute of Technology, 2Department of Bioengineering, California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering, Massachusetts Institute of Technology, 4School of Physics and Astronomy, University of Minnesota
* These authors contributed equally

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Denne fem-dagers protokollen skisserer alle trinn, utstyr, og supplerende programvare som er nødvendig for å skape og drive en effektiv endogen Escherichia coli basert TX-TL celle-fri ytring system fra bunnen av. Med reagenser, tar protokollen 8 timer eller mindre å sette opp en reaksjon, samle inn og behandle data.

Tags

Cellular Biology bioteknologi syntetisk biologi kjemi teknikker Syntetisk molekylærbiologi kontroll teori TX-TL celle-ytringsfrihet in vitro transkripsjon-oversettelse celle-fri protein syntese syntetisk biologi systembiologi Escherichia coli celle ekstrakt biologiske kretser biomolekylære brødfjel
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