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Neuroscience

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用于可视化神经突在冷冻水合状态下使用低温电子断层扫描准备初级神经元
 
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用于可视化神经突在冷冻水合状态下使用低温电子断层扫描准备初级神经元

Article DOI: 10.3791/50783-v 09:59 min February 12th, 2014
February 12th, 2014

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Summary

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Click here for the English version.

以保留神经流程超微结构分析,我们描述了一种协议,用于在电子显微镜网格后用快速冷冻原代神经元的电镀,得到的样品悬浮在一层玻璃的冰。这些样本可以检查与一个低温电子显微镜可视化结构在纳米尺度。

Tags

神经科学,第84,神经元,低温电子显微镜,电子显微镜体层摄影术,脑,大鼠,初级神经元培养,形态学分析
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