Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Udarbejdelse af Primary neuroner til…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Udarbejdelse af Primary neuroner til visualisering neurites i frossen hydreret tilstand vha. Cryo-Electron Tomography
In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility
Journal (Neuroscience)
In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
Journal (Neuroscience)
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
 
Click here for the English version

Udarbejdelse af Primary neuroner til visualisering neurites i frossen hydreret tilstand vha. Cryo-Electron Tomography

Article DOI: 10.3791/50783-v • 09:59 min • February 12th, 2014
February 12th, 2014 •
1, 2, 3, 4, 2, 3, 4
1Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Neuroscience, Baylor College of Medicine, 3Department of Neuroscience, University of California at San Diego, 4National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

For at bevare neuronale processer til ultrastrukturelle analyse beskriver vi en protokol til galvanisering af primære neuroner på elektronmikroskopi gitre efterfulgt af flash frysning, hvilket giver prøver suspenderet i et lag af glasagtig is. Disse prøver kan undersøges med en cryo-elektron mikroskop til at visualisere strukturer på nanometer skala.

Tags

Neuroscience neuroner Cryo-elektronmikroskopi Electron Microscope Tomography Brain rotte primær neuron kultur morfologisk analyse
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter