Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

Calcium Phosphate Transfection of Primary Hippocampal Neurons
 

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Article DOI: 10.3791/50808-v 08:22 min November 12th, 2013
November 12th, 2013

Chapters

Summary

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

Tags

Calcium Phosphate Precipitation Transfection Primary Hippocampal Neurons Cultured Cells Hard-to-transfect Cells Protocol Astroglial Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter