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JoVE Journal
Bioengineering

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膜脊柱:一个生化工具来确定膜蛋白的蛋白质 - 蛋白质相互作用
 
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膜脊柱:一个生化工具来确定膜蛋白的蛋白质 - 蛋白质相互作用

Article DOI: 10.3791/50810-v 10:58 min November 7th, 2013
November 7th, 2013

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Summary

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一个生化方法描述识别体内蛋白质膜蛋白的相互作用(PPI)。该方法结合了蛋白质的交联,亲和纯化和质谱法,并且是适合于几乎任何类型的细胞或生物体。用这种方法,瞬态质子泵抑制剂的甚至识别成为可能。

Tags

生物工程,81期,膜蛋白,
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