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JoVE Journal Chemistry
Kovalent binding af BMP-2 på overflader med en selv-samlet monolag Approach
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Kovalent binding af BMP-2 på overflader med en selv-samlet monolag Approach

Article DOI: 10.3791/50842-v • 10:23 min • August 26th, 2013
August 26th, 2013 •
1, 1, 1,2
1Department of Biophysical Chemistry, Institute for Physical Chemistry, University of Heidelberg, 2Max Planck Institute for Intelligent Systems at Stuttgart

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Vi beskriver en metode til opstilling af en effektiv immobilisering af BMP-2 på overflader. Vores tilgang er baseret på dannelsen af ​​en selvstændig samlet monolag for at opnå den kovalente binding af BMP-2 via dets frie aminrester. Denne metode er et nyttigt værktøj til at studere signalering på cellemembranen.

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Kemi Biokemi Kemiteknik Bioengineering Biomedical Engineering Biofysik Genetik Chemical Biology Physical Chemistry Proteiner biovidenskab biologiske faktorer kemi og materialeteknologi (Generelt) knoglemorfogenprotein 2 (BMP-2) selv-samlet monolag (SAM) kovalent immobilisering NHS-linker BMP-2 signalering protein-assay
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