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JoVE Journal
Immunology and Infection

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Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response
 

Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response

Article DOI: 10.3791/50892-v 07:49 min December 4th, 2013
December 4th, 2013

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Summary

Levels of the inflammatory cell-signaling molecule nitric oxide (NO) are commonly assayed using Griess reagent. In this protocol, we have created a modified Griess assay utilizing live Drosophila brain tissue in order to detect the secretion of NO in a simple, quantifiable and highly repeatable method.

Tags

Novel Whole-tissue Quantitative Assay Nitric Oxide Levels Drosophila Neuroinflammatory Response Neuroinflammation Innate Immune Response Central Nervous System Neuron Damage Infection Immune Infractions Immune Cells Pathology Neurodegenerative Disorders Parkinson's Disease Alzheimer's Disease ALS Chronic Inflammation Disease Progression Dopaminergic Neurons Midbrain Environmental Toxins Sporadic Cases Herbicide Paraquat Animal Models
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