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JoVE Journal Neuroscience
In-vivo-Elektroporation und Postnatale Zeitraffer-Imaging Neuroblast von Migration in Maus Akute Hirnschnitten
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In-vivo-Elektroporation und Postnatale Zeitraffer-Imaging Neuroblast von Migration in Maus Akute Hirnschnitten

Article DOI: 10.3791/50905-v • 10:51 min • November 25th, 2013
November 25th, 2013 •
*1, *1, 2, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
* These authors contributed equally

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Neuroblast Migration ist ein grundlegendes Ereignis in der postnatalen Neurogenese. Wir beschreiben ein Protokoll für die effiziente Markierung von Neuroblasten durch in vivo Elektroporation postnatalen und anschließende Visualisierung der Migration mit Zeitraffer-Bildgebung der akuten Hirnschnitten. Wir sind eine Beschreibung für die quantitative Analyse von Neuroblast Dynamik von Video-Tracking.

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Neuroscience Ausgabe 81 Time-Lapse-Imaging Zellmigrationsassays Elektroporation Neurogenese Neuroblast Migration neurale Stammzellen Subventrikularzone (SVZ) rostralen Migrationsstrom (RMS) neonatalen Maus Welpen Elektroporation Zeitraffer-Bildgebung Gehirn Schnittkultur Zellverfolgung
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