观察和量化成纤维细胞介导的​​纤维蛋白胶压实

Bioengineering

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De Jesús, A. M., Sander, E. A. Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction. J. Vis. Exp. (83), e50918, doi:10.3791/50918 (2014).

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Abstract

Materials

Name Company Catalog Number Comments
Sigma-Aldrich F8630
Sigma-Aldrich T4648
Gibco 11965-092
Gibco 15140-122
Sigma-Aldrich A2942
Sigma-Aldrich H0887
Sigma-Aldrich 223506
Gibco 25200-056
Invitrogen 3000
Lonza DE14-701F
Molecular Probes F8858
GIBCO A10483-01
GIBCO 11430-030
Fisher-Scientific SS264-1
Sigma-Aldrich A3428-25MG
Biotense Bioreactor ADMET
Ti-Eclipe Microscope Nikon
# 0 35 mm Glass Bottom Petri Dish MatTek P35G-0-20-C
# 0 35 mm Glass Top Petri Dish MatTek P35GTOP-0-20-C
Plastic Luer fittings, PVC tubing with Luer ends Cole-Parmer 30600-65

DOWNLOAD MATERIALS LIST

References

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