De células inteiras MALDI-TOF espectrometria de massa é um método rápido e preciso para analisar os diferentes modos de ativação de macrófagos

Immunology and Infection

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Ouedraogo, R., Daumas, A., Capo, C., Mege, J. L., Textoris, J. Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation. J. Vis. Exp. (82), e50926, doi:10.3791/50926 (2013).

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Abstract

MALDI-TOF é uma técnica de espectrometria de massa usados ​​extensivamente na química e bioquímica. Tem sido também aplicado na medicina para identificar moléculas e biomarcadores. Recentemente, tem sido utilizado em microbiologia para identificação rotineira de bactérias cultivadas a partir de amostras clínicas, sem preparação ou fraccionamento passos. Nós e outros autores aplicada esta célula inteira de massa MALDI-TOF técnica de espectrometria com sucesso para células eucarióticas. As aplicações atuais variam de identificação do tipo de célula para avaliação da cultura de células e aplicações de diagnóstico de controle de qualidade. Aqui, descrevemos seu uso para explorar os vários fenótipos de polarização de macrófagos em resposta a citocinas ou bactérias mortas pelo calor. Ele permitiu a identificação de impressões digitais específicos de macrófagos, que são representativas da diversidade de respostas proteômica de macrófagos. Esta aplicação ilustra a precisão e simplicidade do método. O protocolo que descrevemos aqui pode ser útil for estudar a resposta imune do hospedeiro, em condições patológicas, ou pode ser estendida a aplicações de diagnóstico mais amplas.

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