Ganzzell MALDI-TOF-Massenspektrometrie ist eine genaue und schnelle Methode, um verschiedene Arten der Makrophagen-Aktivierung Analysieren

Immunology and Infection

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Ouedraogo, R., Daumas, A., Capo, C., Mege, J. L., Textoris, J. Whole-cell MALDI-TOF Mass Spectrometry is an Accurate and Rapid Method to Analyze Different Modes of Macrophage Activation. J. Vis. Exp. (82), e50926, doi:10.3791/50926 (2013).

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Abstract

MALDI-TOF ist eine extensiv genutzte Massenspektrometrie-Technik in der Chemie und Biochemie. Es wurde auch in der Medizin, um Moleküle und Biomarker zu identifizieren. Kürzlich wurde in der Mikrobiologie für die Routine Identifizierung von Bakterien aus klinischen Proben verwendet worden gewachsen, ohne Vorbereitung oder Fraktionierungsstufen. Wir und andere haben diese Ganzzell-MALDI-TOF-Massenspektrometrie-Technik erfolgreich in eukaryotischen Zellen angewendet. Aktuelle Anwendungen reichen von Zelltyp zu Identifikations Bewertung der Qualitätskontrolle von Zellkultur-und Diagnoseanwendungen. Hier beschreiben wir die Anwendung auf die verschiedenen Phänotypen Polarisierung von Makrophagen in Reaktion auf Cytokine oder Hitze abgetöteten Bakterien erforschen. Es erlaubt die Identifizierung von Makrophagen-spezifischen Fingerabdrücke, die repräsentativ für die Vielfalt der proteomischen Reaktionen von Makrophagen sind. Diese Anwendung stellt die Genauigkeit und Einfachheit des Verfahrens. Das Protokoll wir hier beschrieben kann sinnvoll sein, for das Studium der Immunantwort im Wirt pathologischen Bedingungen oder breitere diagnostische Anwendungen erweitert werden.

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