Murine महाधमनी साइनस में atherosclerotic घावों की मात्रात्मक विश्लेषण और विशेषता

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Venegas-Pino, D. E., Banko, N., Khan, M. I., Shi, Y., Werstuck, G. H. Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus. J. Vis. Exp. (82), e50933, doi:10.3791/50933 (2013).

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Abstract

Materials

Name Company Catalog Number Comments
LDLR-deficient mice (D2.129S7(B6)-Ldlrtm1Her/J) The Jackson Laboratory stock #002207
Formalin Sigma-Aldrich HT5011
Histology Molds Ted Pella Inc 27195
Paraffin Paramat 19286-10
Coated slides Fisher 12-550-15
Solvent containers Tissue Tek II 4457
Microtome Leica RM22S5
Water bath VWR 80086-982
Microscope Olympus BX41TF
Camera Olympus DP72
ImageJ NIH www.rsbweb.nih.gov/ij/
Processor Sakura Finetek VIP6-A1
Processing cassettes VWR 18000-134
Preassure Cooker Nordic Ware
Coverslip Fisher 12-545-M
PBS Sigma-Aldrich P3813
Microtome blade Thermo Scientific 30-518-35
Antifade mounting medium Sigma-Aldrich F-4680
Xylene mounting medium  Sigma-Aldrich 44581
Aquous mounting medium Sigma-Aldrich I1161
Mac-3 anbody BD Pharmingen 553322
DAPI Sigma-Aldrich D9542
F4/80 antibody Abcam Ab6640
Alpha actin antibody Santa Cruz Biotechnology SC-32251
Mayer’s Hematoxylin Sigma-Aldrich E4382
Eosin Y Sigma-Aldrich MHS16
DAB (3,3'-Diaminobenzidine) Dako K3468

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References

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