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JoVE Journal Bioengineering
Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier
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Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier

Article DOI: 10.3791/50934-v • 08:40 min • November 12th, 2013
November 12th, 2013 •
1, 1
1Australian Centre for Blood Diseases, Monash University

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Summary
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Establishment of human models of the blood-brain barrier (BBB) can benefit research into brain conditions associated with BBB failure. We describe here an improved technique for preparation of a contact BBB model, which permits coculturing of human astrocytes and brain endothelial cells on the opposite sides of a porous membrane.

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Blood-brain Barrier BBB Human Cell-based Model Contact Model In Vitro Model Brain Pathologies Astrocytes Brain Endothelial Cells Coculture Porous Membrane High Throughput Screening Primary Human BECs Immortalized Human Astrocytes Cell-seeding Technique Insert Staining Techniques BBB-related Research
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