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JoVE Journal Bioengineering
Forbedret metode til forberedelse af en menneskelig celle-baseret, Contact Model af blod-hjerne barrieren
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Forbedret metode til forberedelse af en menneskelig celle-baseret, Contact Model af blod-hjerne barrieren

Article DOI: 10.3791/50934-v • 08:40 min • November 12th, 2013
November 12th, 2013 •
1, 1
1Australian Centre for Blood Diseases, Monash University

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Etablering af humane modeller af blod-hjerne-barrieren (BBB) ​​kan gavne forskning i hjernesygdomme forbundet med BBB fiasko. Vi beskriver her en forbedret teknik til fremstilling af en kontakt BBB model, som tillader coculturing menneskelige astrocytter og hjerne endotelceller på de modstående sider af en porøs membran.

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Bioteknik blod-hjerne barrieren model cellekultur astrocytter hjerne endotelceller insert membraner
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