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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolamento di cellule dendritiche mieloidi e cellule epiteliali di timo umano
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Isolamento di cellule dendritiche mieloidi e cellule epiteliali di timo umano

Article DOI: 10.3791/50951-v • 09:07 min • September 19th, 2013
September 19th, 2013 •
1,2, 1, 3, 4, 5, 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

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Dettagli Questo protocollo un metodo per isolare le cellule presentanti l'antigene da timo umano attraverso diverse fasi di digestione enzimatica del tessuto seguita dalla densità centrifugazione della sospensione di cellule singole e smistamento finalmente magnetico e / o FACS delle popolazioni cellulari di interesse.

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Immunologia Numero 79 Processi del sistema immunitario i processi biologici immunologia malattie del sistema immunitario sistema immunitario Fenomeno Life Sciences (Generale) immunologia timo umano l'isolamento le cellule dendritiche MTEC CTEC
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