Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
 
Click here for the English version

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Article DOI: 10.3791/50952-v 09:10 min December 16th, 2013
December 16th, 2013

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host mouse.

Tags

Lymph Node Fat Pad Chimeras Stromal Cells Origin Cellular Composition Mechanisms Adipose Tissue Adipocyte Precursor Cells Lymphoid Stromal Cells Lineage Tracing Newborn Lymph Nodes EYFP+ Embryonic Adipose Tissue LN/EYFP+ Fat Pad Chimera Kidney Capsule Transfer Progeny Analysis Flow-cytometric Analysis Enzymatically Digested Lymph Nodes Immunofluorescence Analysis Knockout Mouse Models Cell Population
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter