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Journal / Neuroscience / Nucleofection di roditori neuroblasti per…
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Neuroscience

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JoVE Journal Neuroscience
Nucleofection di roditori neuroblasti per studiare neuroblasti migrazione
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Nucleofection di roditori neuroblasti per studiare neuroblasti migrazione

Article DOI: 10.3791/50989-v • 11:32 min • November 12th, 2013
November 12th, 2013 •
*1, *2, 1, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London
* These authors contributed equally

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Migrazione neuroblasti è un passo fondamentale nella neurogenesi postnatale. Il protocollo qui descritto può essere utilizzato per studiare il ruolo dei regolatori candidati migrazione neuroblasti impiegando DNA / piccolo tornante RNA (shRNA) nucleofection e un saggio di migrazione 3D con neuroblasti isolati dal flusso migratorio rostrale roditore postnatale.

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Neuroscienze Numero 81 Biologia Cellulare Cellulari migrazione saggi Trasfezione neurogenesi zona subventricolare (SVZ) le cellule staminali neurali flusso migratorio rostrale (RMS) neuroblast 3D test di migrazione nucleofection
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