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Journal / Neuroscience / Nucleofection de roedores Neuroblastos para…
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Neuroscience

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JoVE Journal Neuroscience
Nucleofection de roedores Neuroblastos para estudar neuroblasto Migração
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Nucleofection de roedores Neuroblastos para estudar neuroblasto Migração

Article DOI: 10.3791/50989-v • 11:32 min • November 12th, 2013
November 12th, 2013 •
*1, *2, 1, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London
* These authors contributed equally

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Migração neuroblastos é um passo crucial na neurogênese pós-natal. O protocolo aqui descrito pode ser utilizado para investigar o papel dos reguladores de candidatos de migração neuroblastos empregando DNA / RNA pequeno hairpin (shRNA) nucleofection e um ensaio de migração 3D com neuroblastos isolados do roedor pós-natal fluxo migratório rostral.

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Neurociência Edição 81 Biologia Celular Celulares Ensaios de Migração transfecção neurogênese zona subventricular (SVZ) as células-tronco neurais fluxo migratório rostral (RMS) neuroblastos ensaio de migração em 3D nucleofection
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