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Journal / Neuroscience / Kemirgen neuroblasts nucleofection Neuroblast Göç …
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Neuroscience

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JoVE Journal Neuroscience
Kemirgen neuroblasts nucleofection Neuroblast Göç Eğitim için
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Kemirgen neuroblasts nucleofection Neuroblast Göç Eğitim için

Article DOI: 10.3791/50989-v • 11:32 min • November 12th, 2013
November 12th, 2013 •
*1, *2, 1, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London
* These authors contributed equally

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Neuroblast göç postnatal nörojenezindeki çok önemli bir adımdır. Protokol Burada açıklanan DNA / küçük firkete RNA (ShRNA) nucleofection ve kemirgen doğum sonrası rostral göçmen akımından izole nöroblast ile 3D göç deneyi kullanılarak neuroblast göç aday düzenleyicilerinin rolünü araştırmak için kullanılabilir.

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Nörobilim Sayı 81 Hücresel Biyoloji Hücre Göç Tahliller Transfeksiyon nöron bölge subventricular (SVZ) nöral kök hücreler rostral göçmen akışı (RMS) neuroblast 3D göç deneyi nucleofection
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