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Bioengineering

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JoVE Journal Bioengineering
Met behulp van Microfluidics Chips voor Live Imaging en Studie van Injury Reacties in
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Met behulp van Microfluidics Chips voor Live Imaging en Studie van Injury Reacties in

Article DOI: 10.3791/50998-v • 11:46 min • February 7th, 2014
February 7th, 2014 •
1, 2, 1, 1, 1, 3,4, 2,5, 1
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 2Department of Biomedical Engineering, University of Michigan, 3Life Sciences Institute, University of Michigan, 4Department of Cell and Developmental Biology, University of Michigan, 5Department of Mechanical Engineering, University of Michigan

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Drosophila larven zijn een aantrekkelijk model systeem voor live-imaging vanwege hun doorschijnende cuticula en krachtige genetica. Dit protocol beschrijft hoe u een single-layer PDMS apparaat, genaamd de 'larve chip' voor live-beeldvorming van cellulaire processen binnen neuronen van 3 e instar Drosophila larven benutten.

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Biotechniek , Live Imaging Microfluidics axonalverwonding axonale degeneratie calcium beeldvorming photoconversion laser microchirurgie
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