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Bioengineering

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JoVE Journal Bioengineering
Mit Mikrofluidik-Chips für Live-Imaging-und Studienverletzungs Antworten in
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Mit Mikrofluidik-Chips für Live-Imaging-und Studienverletzungs Antworten in

Article DOI: 10.3791/50998-v • 11:46 min • February 7th, 2014
February 7th, 2014 •
1, 2, 1, 1, 1, 3,4, 2,5, 1
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 2Department of Biomedical Engineering, University of Michigan, 3Life Sciences Institute, University of Michigan, 4Department of Cell and Developmental Biology, University of Michigan, 5Department of Mechanical Engineering, University of Michigan

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Drosophila-Larven sind eine attraktive Modellsystem für Live-Bildgebung aufgrund ihrer schein Kutikula und leistungsfähige Genetik. Dieses Protokoll beschreibt, wie man eine Single-Layer-PDMS-Gerät, genannt die "Larve Chip" für die Live-Darstellung von zellulären Prozessen in Nervenzellen des 3. Larvenstadium Drosophila-Larven nutzen.

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Bioengineering , Live-Imaging Mikrofluidik axonalen Schädigung axonalen Degeneration Calcium Imaging Photo Laser-Mikrochirurgie
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