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Journal / Bioengineering / Isolation und Charakterisierung von chimeric…
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JoVE Journal Bioengineering
Isolation und Charakterisierung von chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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Isolation und Charakterisierung von chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Article DOI: 10.3791/51023-v • 10:33 min • January 8th, 2014
January 8th, 2014 •
1,2, 1,2, 2, 1, 1, 1, 1, 3, 3
1Department of Chemical and Biomolecular Engineering, Ohio University, 2Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, 3Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School

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Im Vergleich zur traditionellen Affinitätschromatographie mit Protein A Agarose Perlen-verpackten Säulen können Protein-A-Membranadsorber die Isolation von Antikörpern und anderen Fc-Fragment-exemittierenden Proteinen im Labormaßstab erheblich beschleunigen. Geeignete Analyse- und Quantifizierungsmethoden können die Proteinverarbeitung weiter beschleunigen, so dass die Isolierung/Charakterisierung an einem Werktag statt an mehr als 20 Arbeitsstunden abgeschlossen werden kann.

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Bioengineering Ausgabe 83 Affinitätschromatographie Membranadsorber Bioseparationen Protein A Galectin-1 Gal-1hFc
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