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Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes
Journal (Biology)
Isolation of Retinal Pigment Epithelial Cells from Guinea Pig Eyes
Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Journal (Biology)
Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
 
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Den

Article DOI: 10.3791/51040-v • 10:57 min • March 11th, 2014
March 11th, 2014 •
1, 1, 1, 1, 1
1Department of Biomedical Engineering, Washington University in St. Louis

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Anvendes cut-open Vaseline hul tilgang til at opnå lav støj optagelser af ioniske og gating strømme fra spændingsafhængige ionkanaler udtrykt i Xenopus oocyter med høj opløsning på hurtige kanal kinetik. Med mindre ændringer kan spændingsklemme fluorometri kobles til cut-åben oocyt protokol.

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Developmental Biology Voltage clamp Cut-åben Oocyte Voltage Clamp fluorometri natriumkanaler ionstrømmene,
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