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JoVE Journal
Bioengineering
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Sample Drift Korrektion Efter 4D Confocal Time-lapse Imaging
 
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Sample Drift Korrektion Efter 4D Confocal Time-lapse Imaging

Article DOI: 10.3791/51086-v 10:05 min April 12th, 2014
April 12th, 2014

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Summary

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Time-lapse mikroskopi tillader visualisering af udviklingsprocesser. Vækst eller afdrift af prøver under billedet erhvervelse reducerer evnen til præcist at følge og måle celle bevægelser under udvikling. Vi beskriver brugen af ​​open source billedbehandling software til at korrigere for tredimensionelle prøve afdrift over tid.

Tags

Bioteknik Image Processing Computer-Assisted zebrafisk mikroskopi Confocal Time-lapse Imaging billedbehandling zebrafisk Confocal Fiji tre-dimensionelle fire-dimensional registrering
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