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Monster Tijdafwijkingscorrectie Na 4D Confocal time-lapse imaging

Article DOI: 10.3791/51086-v • 10:05 min • April 12th, 2014
April 12th, 2014 •
1, 2, 1
1School of Biological Sciences, Monash University, 2Janelia Farm Research Campus, Howard Hughes Medical Institute

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Time-lapse microscopie kan de visualisatie van ontwikkelingsprocessen. Groei of drift van monsters tijdens beeldacquisitie vermindert het vermogen om nauwkeurig te volgen en te meten cel bewegingen tijdens de ontwikkeling. We beschrijven het gebruik van open source software voor beeldverwerking om te corrigeren voor driedimensionale sample drift in de tijd.

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Biotechniek Beeldverwerking Computer-Assisted zebravis microscopie confocale time-lapse imaging beeldvorming zebravis confocale fiji drie-dimensionale vier-dimensionale registratie
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