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JoVE Journal Neuroscience
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
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Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Article DOI: 10.3791/51091-v • 13:22 min • January 23rd, 2014
January 23rd, 2014 •
1,2,5, 4, 4, 1,2,3
1Virus and Centromere Team, Centre de Génétique et Physiologie Moléculaire et Cellulaire, CNRS UMR 5534, 2Université de Lyon 1, 3Laboratoire d'excellence, LabEX DEVweCAN, 4Institut de Virologie Moléculaire et Structurale, CNRS UPR 3296, 5Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR 5286

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Summary
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We established a fluorescent in situ hybridization protocol for the detection of a persistent DNA virus genome within tissue sections of animal models. This protocol enables studying infection process by codetection of the viral genome, its RNA products, and viral or cellular proteins within single cells.

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Genome Detection Detection Persistent DNA Virus Fluorescent In Situ Hybridization Immunostaining Gene Expression Regulation Virology Animal Models Herpes Simplex Virus Type 1 (HSV-1) Latent Infection Viral Genomes Neuronal Tissues DNA-FISH RNA-FISH Immunofluorescence Confocal Microscopy
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