Progettazione e analisi di temperatura Preferenze comportamento e il suo ritmo circadiano in

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Goda, T., Leslie, J. R., Hamada, F. N. Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila. J. Vis. Exp. (83), e51097, doi:10.3791/51097 (2014).

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Abstract

Materials

Name Company Catalog Number Comments
Bright Lab Jr. Safelight Amazon #B00013J8UY Red light for dark rooms
Rain X SOPUS products Water repellent: Apply the plexiglass cover
C-Clamp Home Depot
Temperature/hygrometer Fisher 15-077-963
Peltier devices TE Technology, Inc. HP-127-1.4-1.15-71P
Thermometer Fluke Fluke 52II
Bench top controller Oven Industries 5R6-570-15R and 5R6-570-24R
Temperature sensor probe Oven Industries TR67-32
Generic 480 Watt ATX power supply computer cooling system
MCR220-QP-RES Dual 120 mm Radiator with reservoir  Swiftech computer cooling system
MCP350 In-Line 12V DC pump Swiftech computer cooling system
MCW50 graphics Card liquid cooler Swiftech computer cooling system
Scythe Kaze-Jyuni SY1225SL12SH fan Crazy PC computer cooling system

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References

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  3. Kaneko, H., et al. Circadian Rhythm of Temperature Preference and Its Neural Control in Drosophila. Curr. Biol. 22, 1851-1857 (2012).
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  17. Crocker, A., Sehgal, A. Genetic analysis of sleep. Genes Dev. 24, 1220-1235 (1220).
  18. Hendricks, J. C., et al. Rest in Drosophila is a sleep-like state. Neuron. 25, 129-138 (2000).
  19. Shaw, P. J., Cirelli, C., Greenspan, R. J., Tononi, G. Correlates of sleep and waking in Drosophila melanogaster. Science. 287, 1834-1837 (2000).
  20. Parisky, K. M., et al. PDF cells are a GABA-responsive wake-promoting component of the Drosophila sleep circuit. Neuron. 60, 672-682 (2008).

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