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Journal / Immunology and Infection / Visualiseringen af ​​det immunologiske Synapse…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualiseringen af ​​det immunologiske Synapse med Dual Color Time-gated stimuleret emission Nedbrydning (STED) Nanoscopy
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Visualiseringen af ​​det immunologiske Synapse med Dual Color Time-gated stimuleret emission Nedbrydning (STED) Nanoscopy

Article DOI: 10.3791/51100-v • 10:00 min • March 24th, 2014
March 24th, 2014 •
1, 1
1Center for Human Immunobiology, Texas Children's Hospital and Baylor College of Medicine

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Her illustrerer vi protokollen for billeddannelse med tofarvede STED Nanoscopy den cytotoksiske immun synapse af NK-celler sammenfattet på glas. Ved hjælp af denne metode, vi får sub-100 nm opløsning på synapse proteiner og cytoskelettet.

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Immunologi naturlige dræberceller F-actin immun synapse super-opløsning mikroskopi tofarvet stimuleret emission depletion (STED) mikroskopi
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