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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisatie van de immunologische Synapse door Dual Color Time-gated stimulated emission depletion (STED) Nanoscopy
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Visualisatie van de immunologische Synapse door Dual Color Time-gated stimulated emission depletion (STED) Nanoscopy

Article DOI: 10.3791/51100-v • 10:00 min • March 24th, 2014
March 24th, 2014 •
1, 1
1Center for Human Immunobiology, Texas Children's Hospital and Baylor College of Medicine

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Hier illustreren we het protocol voor beeldvorming door twee kleuren STED nanoscopy de cytotoxische immuun synaps van NK-cellen samengevat op glas. Met deze methode krijgen we sub-100 nm resolutie van synapseiwitten en het cytoskelet.

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Immunologie natural killer cellen F-actine immuun synaps super-resolutie microscopie twee kleuren stimulated emission depletion (STED) microscopie
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