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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualização do imunológica Synapse por Dual Color Time-fechado Emissão Estimulada Exaustão (STED) Nanoscopia
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Visualização do imunológica Synapse por Dual Color Time-fechado Emissão Estimulada Exaustão (STED) Nanoscopia

Article DOI: 10.3791/51100-v • 10:00 min • March 24th, 2014
March 24th, 2014 •
1, 1
1Center for Human Immunobiology, Texas Children's Hospital and Baylor College of Medicine

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Aqui, ilustramos o protocolo para a imagiologia por duas cores STED Nanoscopia citotóxica sinapse imune das células NK retomados em vidro. Usando este método, obtemos resolução nm sub-100 de proteínas de sinapses e do citoesqueleto.

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Imunologia Edição 85 as células matadoras naturais F-actina sinapse imunológica microscopia de super-resolução duas cores estimulada esgotamento emissão microscopia (STED)
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