Клеточная проточной цитометрии измерить цитотоксическую активность

Immunology and Infection

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Noto, A., Ngauv, P., Trautmann, L. Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity. J. Vis. Exp. (82), e51105, doi:10.3791/51105 (2013).

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Name Company Catalog Number Comments
EasySep Human CD4+ T Cell Enrichment Kit Stem Cell 19052
EasySep Human CD8+ T Cell Enrichment Kit Stem Cell 19053
CellTrace CFSE Cell Proliferation Kit - For Flow Cytometry Invitrogen C34554
Far Red LIVE/DEAD fixable dead cell Invitrogen L10120
Alexa Fluor 700 Mouse Anti-Human CD3 BD 557943
Brilliant Violet 650 Anti-Human CD4 Antibody Biolegend 317435
PerCP anti-human CD8 Antibody Biolegend 344707
RPMI 1640 Medium 1x VWR 10-040-CV
Penicillin-Streptomycin 100x Solution Invitrogen 15070063
Fetal Bovine Serum - Gold, Heat Inactivated PAA A15-252
96-well plates; Treated; U-bottom; with lid BD Falcon 353077
25 ml sterile polystyrene, individually wrapped reservoirs USA Scientific 2321-2230
96-well U-bottom, sterile 2 ml deep well plates Thermo Scientific 275743
Human IL-2 (v126), research grade Miltenyi Biotec 130-093-096
RoboSep Buffer, separation buffer Stem cell 20104
15 ml Polypropylene Conical Tube BD Falcon 352097
14 ml round bottom tube, polypropylene BD Falcon 352059
The Big Easy EasySep Magnet Stem cell 18001
PBS 1x VWR 21-040-CV
96-well; V-bottom plate with lid Fisher 12-565-481
Formaldehyde solution, ACS reagent, 37 wt % in H2O Sigma-Aldrich 252549-500ML
1.5 ml screw cap microtube, conical Sarstedt 72.692.005



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