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Journal / Immunology and Infection / Expressão da Funcionais recombinante…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Expressão da Funcionais recombinante hemaglutinina ea neuraminidase Proteínas do vírus Influenza H7N9 Novel usando a expressão Sistema de Baculovirus
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Expressão da Funcionais recombinante hemaglutinina ea neuraminidase Proteínas do vírus Influenza H7N9 Novel usando a expressão Sistema de Baculovirus

Article DOI: 10.3791/51112-v • 12:18 min • November 6th, 2013
November 6th, 2013 •
1,2, 1,3, 1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Medicine, Icahn School of Medicine at Mount Sinai

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Aqui nós descrevemos uma forma de expressar antígenos de superfície do vírus da influenza corretamente dobradas e funcionais decorrentes do novo vírus H7N9 chinês em células de insetos. A técnica pode ser adaptado para expressar ectodomínios de quaisquer proteínas de superfície virais ou celulares.

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Infecção o vírus da Influenza A, Influenza Aviária Vacinas contra Influenza a hemaglutinina neuraminidase H7N9 baculovírus células de inseto a expressão da proteína recombinante
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