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JoVE Journal Biology
Dissecção
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Dissecção

Article DOI: 10.3791/51118-v • 09:07 min • March 4th, 2014
March 4th, 2014 •
1,2,3,4, 1,2,3,4
1Institut Curie, Centre Universitaire, 2Université Paris Sud, Centre Universitaire, 3CNRS UMR 3347, Centre Universitaire, 4INSERM U1021, Centre Universitaire

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Este protocolo descreve como dissecar crista neural craniana premigratory (NC) de Xenopus laevis neurulas. Estes explantes podem ser semeadas em fibronectina e cultivadas in vitro, ou enxertados em embriões hospedeiros. Esta técnica permite estudar os mecanismos da transição epitélio NC-to-mesênquima, migração e diferenciação.

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Biologia do Desenvolvimento edição 85 da crista neural, Embrião dissecção concussão fibronectina
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