Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Using Fluorescent Proteins to Visualize and…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
Journal (Immunology and Infection)
Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
Journal (Immunology and Infection)
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
 

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Article DOI: 10.3791/51131-v • 07:42 min • October 13th, 2015
October 13th, 2015 •
1,2, 2, 1
1Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, 2Program in Infectious Diseases, School of Public Health, University of California at Berkeley

Chapters

Summary
Automatic Translation

A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

Tags

Fluorescent Proteins Chlamydia Vacuole Growth Dynamics Living Cells Obligate Intracellular Bacterium Chlamydia Elicits Global Public Health Sexually Transmitted Infection Preventable Blindness Host Cell Signaling Novel Vacuolar Compartment Inclusion Developmental Growth Effector Proteins Cytosolic Face Vacuole Membrane Host Cytosol Visualizing Intracellular Compartment Reverse-imaging Strategy GFP Expressing Host Cells MCherry Expressing Host Cells Antibody-based Enumeration Methods
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter