Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

细胞表面和内蛋白质的现场培养神经细胞的抗体后喂养差异化标记
 
Click here for the English version

细胞表面和内蛋白质的现场培养神经细胞的抗体后喂养差异化标记

Article DOI: 10.3791/51139-v 11:56 min February 12th, 2014
February 12th, 2014

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

我们描述了一种方法,以使用特定的多克隆抗体对表位的细胞外活神经元的表面上的标记蛋白。蛋白结合的抗体在细胞表面上,并随后通过胞吞作用内在化可以从蛋白质上残留进行区分,或在培养过程中贩卖到,该表面。

Tags

神经科学,第84,双色荧光免疫细胞化学,贩运,内吞作用,循环内涵体,神经元
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter