Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Methodology for the Efficient Generation of…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
Journal (Immunology and Infection)
Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
Journal (Immunology and Infection)
Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
 

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Article DOI: 10.3791/51151-v • 09:27 min • January 17th, 2014
January 17th, 2014 •
*1, *1, 1, 2, 3, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Medicine, Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science, University of Melbourne
* These authors contributed equally

Chapters

Summary
Automatic Translation

A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

Tags

Methodology Efficient Generation Fluorescently Tagged Vaccinia Virus Proteins Tagging Viral Proteins Fluorescent Proteins Virus-host Interactions Live-cell Microscopy Recombinant Viruses Homologous Recombination Construct Generation Transient Dominant Selection Fluorescent Reporter Metabolic Selection Rapid Approach Modular Approach Labeling Viral Proteins Downstream Applications Advanced Imaging Analysis Virus-host Interplay Virus Replication
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter