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Journal / Biology / Snabb Syntes och Screening av kemiskt aktiverade…
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Biology

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JoVE Journal Biology
Snabb Syntes och Screening av kemiskt aktiverade transkriptionsfaktorer med GFP-baserade Reportrar
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Snabb Syntes och Screening av kemiskt aktiverade transkriptionsfaktorer med GFP-baserade Reportrar

Article DOI: 10.3791/51153-v • 09:22 min • November 26th, 2013
November 26th, 2013 •
*1,3, *1, 1,2, 1
1The Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Department of Molecular Biology, Princeton University, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

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Detta protokoll beskriver en experimentell procedur för snabb konstruktion av artificiella transkriptionsfaktorer (atfs) med besläktade GFP reportrar och kvantifiering av atfs förmåga att stimulera GFP uttryck via flödescytometri.

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Genetik transkription transkriptionsfaktorer artificiella transkriptionsfaktorer zinkfingrar Zif268 syntetisk biologi
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