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Murray, L., Gillingwater, T. H., Kothary, R. Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis. J. Vis. Exp. (83), e51162, doi:10.3791/51162 (2014).

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Name Company Catalog Number Comments
Paraformaldehyde Aqueous Solution (16% ) Electron Micropscopy Sciences 15700
Dumont #5 Forceps Fine Science Tools 11251-10
Angled Sprung Scissors Fine Science Tools 15006-09
Fine Scissors - ToughCut Fine Science Tools 14058-09
SYLGARD 184 Silicone Elastomer Kit Dow Corning dependant on local supplier Use this to make dissection dish for pinning out muscle
Minutien Pins Fine science tools 26002-20
α-Bungarotoxin, Alexa Fluor 488 Conjugate Invitrogen B-13422
Albumin from Bovine Serum Sigma Aldrich A4503
Neurofilament Primary antibody (2H3), Supernatant Developmental Studies Hybridoma Bank
SV2 Primary antibody (SV2), Supernatant Developmental Studies Hybridoma Bank
Goat Anti-Mouse IgG (H+L) Jackson ImmunoResearch 115-166-003
Fluorescence Mounting Medium Dako S3023
Slides (Superfrost Plus; White) Fisher 12-550-15
Coverslips Fisher
Triton X-100 Sigma Aldrich T8787
CD1/C57Bl6 mouse Jackson Labs



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