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JoVE Journal
Immunology and Infection

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Vurdere udviklingen i murine Plasmacytoid dendritiske celler i Peyer'ske Patches Brug adoptiv overførsel af Hematopoietic Progenitorer
 
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Vurdere udviklingen i murine Plasmacytoid dendritiske celler i Peyer'ske Patches Brug adoptiv overførsel af Hematopoietic Progenitorer

Article DOI: 10.3791/51189-v 13:34 min March 17th, 2014
March 17th, 2014

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Summary

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Denne protokol beskriver eksperimentelle procedurer til at vurdere differentiering af plasmacytoid dendritiske celler i Peyer patch fra fælles dendritiske celle stamceller ved hjælp af teknikker, der involverer FACS-medieret celle isolation, hydrodynamisk genoverførsel, og flow analyse af immun delmængder i Peyers patch.

Tags

Immunologi hæmatopoiesis dendritiske celler Peyer patch cytokiner adoptiv overførsel
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